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γ-(6-aminohexyl)-atp-cy5  (Jena Bioscience)


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    Structured Review

    Jena Bioscience γ-(6-aminohexyl)-atp-cy5
    γ (6 Aminohexyl) Atp Cy5, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cy5/%CE%B3-(6-Aminohexyl)-ATP-Cy5/custom%40nu-833-cy5%4042703972
    Average 92 stars, based on 2 article reviews
    γ-(6-aminohexyl)-atp-cy5 - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Purification:

    Article Title: Dynamic patterns of repeats and retrotransposons in the centromeres of Humulus lupulus L.
    Article Snippet: PCR products were separated on a 1% agarose gel with EtBr staining and purified using the QIAquick PCR Purification Kit (28104; Qiagen). .. Purified DNA (1 μg) was labeled with Atto488 NT (PP‐305L‐488), Atto550 NT (PP‐305L‐550) or Cy5 (PP‐305L‐647N) using Nick translation labeling kits (Jena Bioscience, Jena, Germany) following the manufacturer's instructions. ..

    Labeling:

    Article Title: Dynamic patterns of repeats and retrotransposons in the centromeres of Humulus lupulus L.
    Article Snippet: PCR products were separated on a 1% agarose gel with EtBr staining and purified using the QIAquick PCR Purification Kit (28104; Qiagen). .. Purified DNA (1 μg) was labeled with Atto488 NT (PP‐305L‐488), Atto550 NT (PP‐305L‐550) or Cy5 (PP‐305L‐647N) using Nick translation labeling kits (Jena Bioscience, Jena, Germany) following the manufacturer's instructions. ..

    Article Title: Controlling intermolecular base pairing in Drosophila germ granules by mRNA folding and its implications in fly development.
    Article Snippet: .. To detect intermolecular base pairing by RNA gel electrophoresis, 16 pmol of each Cy5 (Jena Bioscience: NU-821-Cy5) labeled shu-top and shu-bottom RNAs were used following the protocol described for in vitro intermolecular RNA base pairing assays and gel electrophoresis. .. After the electrophoresis was complete, the gel was stained with 5μM DFHBI-1T at RT for 15mins and then imaged using AmershamTM TyphoonTM 5 scanner (GE Healthcare) with 488 and Cy5 filters as described before46.

    Article Title: Controlling intermolecular base pairing in Drosophila germ granules by mRNA folding and its implications in fly development
    Article Snippet: .. To detect intermolecular base pairing by RNA gel electrophoresis, 16 pmol of each Cy5 (Jena Bioscience: NU-821-Cy5) labeled shu-top and shu-bottom RNAs were used following the protocol described for in vitro intermolecular RNA base pairing assays and gel electrophoresis. .. After the electrophoresis was complete, the gel was stained with 5 μM DFHBI-1T at RT for 15 mins and then imaged using AmershamTM TyphoonTM 5 scanner (GE Healthcare) with 488 and Cy5 filters as described before .

    Nick Translation:

    Article Title: Dynamic patterns of repeats and retrotransposons in the centromeres of Humulus lupulus L.
    Article Snippet: PCR products were separated on a 1% agarose gel with EtBr staining and purified using the QIAquick PCR Purification Kit (28104; Qiagen). .. Purified DNA (1 μg) was labeled with Atto488 NT (PP‐305L‐488), Atto550 NT (PP‐305L‐550) or Cy5 (PP‐305L‐647N) using Nick translation labeling kits (Jena Bioscience, Jena, Germany) following the manufacturer's instructions. ..

    Nucleic Acid Electrophoresis:

    Article Title: Controlling intermolecular base pairing in Drosophila germ granules by mRNA folding and its implications in fly development.
    Article Snippet: .. To detect intermolecular base pairing by RNA gel electrophoresis, 16 pmol of each Cy5 (Jena Bioscience: NU-821-Cy5) labeled shu-top and shu-bottom RNAs were used following the protocol described for in vitro intermolecular RNA base pairing assays and gel electrophoresis. .. After the electrophoresis was complete, the gel was stained with 5μM DFHBI-1T at RT for 15mins and then imaged using AmershamTM TyphoonTM 5 scanner (GE Healthcare) with 488 and Cy5 filters as described before46.

    Article Title: Controlling intermolecular base pairing in Drosophila germ granules by mRNA folding and its implications in fly development
    Article Snippet: .. To detect intermolecular base pairing by RNA gel electrophoresis, 16 pmol of each Cy5 (Jena Bioscience: NU-821-Cy5) labeled shu-top and shu-bottom RNAs were used following the protocol described for in vitro intermolecular RNA base pairing assays and gel electrophoresis. .. After the electrophoresis was complete, the gel was stained with 5 μM DFHBI-1T at RT for 15 mins and then imaged using AmershamTM TyphoonTM 5 scanner (GE Healthcare) with 488 and Cy5 filters as described before .

    In Vitro:

    Article Title: Controlling intermolecular base pairing in Drosophila germ granules by mRNA folding and its implications in fly development.
    Article Snippet: .. To detect intermolecular base pairing by RNA gel electrophoresis, 16 pmol of each Cy5 (Jena Bioscience: NU-821-Cy5) labeled shu-top and shu-bottom RNAs were used following the protocol described for in vitro intermolecular RNA base pairing assays and gel electrophoresis. .. After the electrophoresis was complete, the gel was stained with 5μM DFHBI-1T at RT for 15mins and then imaged using AmershamTM TyphoonTM 5 scanner (GE Healthcare) with 488 and Cy5 filters as described before46.

    Article Title: Controlling intermolecular base pairing in Drosophila germ granules by mRNA folding and its implications in fly development
    Article Snippet: .. To detect intermolecular base pairing by RNA gel electrophoresis, 16 pmol of each Cy5 (Jena Bioscience: NU-821-Cy5) labeled shu-top and shu-bottom RNAs were used following the protocol described for in vitro intermolecular RNA base pairing assays and gel electrophoresis. .. After the electrophoresis was complete, the gel was stained with 5 μM DFHBI-1T at RT for 15 mins and then imaged using AmershamTM TyphoonTM 5 scanner (GE Healthcare) with 488 and Cy5 filters as described before .

    Transfection:

    Article Title: Spatial mapping of DNA synthesis reveals dynamics and geometry of human replication nanostructures.
    Article Snippet: For nucleoside pulse labelling, 5-ethynyl-2′-deoxyuridine (EdU) (Jena Bioscience, CLKN001-100) or 5-ethynyl-arabino-uridine (F-ara-EdU) (Jena Bioscience, CLK-1403-25) was added to the media of live cells at the stated concentration and for the stated amount of time. .. For fluorescent dNTP pulse labelling, Cy3-dUTP, Cy3-xx-dUTP, Cy5dUTP or Cy5-xx-dUTP (Jena Bioscience, NU-803-CY3-S, NU-803XX-CY3-S, NU-803-XX-CY5-S, where ‘xx’ denotes a linker structure between the nucleoside and fluorophore molecule), were mixed with a synthetic nucleoside triphosphate transporter (SNTT1) (6 μM, Sigma-Aldrich, SCT064) as follows: the dNTP and the transporter were diluted in tricine buffer (Kužmová et al, 2021), each diluted component was mixed 1:1 to form the transfection mixture. ..

    Article Title: Spatial mapping of DNA synthesis reveals dynamics and geometry of human replication nanostructures
    Article Snippet: Human bronchial epithelial cells (hereafter referred to as HBEC) CDC6 Tet-On cells, were previously described (Komseli et al, ) and cultured in Keratinocyte-SFM Medium with 5 ng/ml EGF and 50 μg/ml bovine pituitary extract (BPE) (Thermo Fisher Scientific, 10144892), supplemented with 1% penicillin-streptomycin (Thermo Fisher Scientific, 15140122). .. For fluorescent dNTP pulse labelling, Cy3-dUTP, Cy3-xx-dUTP, Cy5-dUTP or Cy5-xx-dUTP (Jena Bioscience, NU-803-CY3-S, NU-803-XX-CY3-S, NU-803-XX-CY5-S, where ‘xx’ denotes a linker structure between the nucleoside and fluorophore molecule), were mixed with a synthetic nucleoside triphosphate transporter (SNTT1) (6 μM, Sigma-Aldrich, SCT064) as follows: the dNTP and the transporter were diluted in tricine buffer (Kužmová et al, ), each diluted component was mixed 1:1 to form the transfection mixture. .. RPE1 cells were seeded on glass cover slides (Thermo Fisher Scientific, 11846933) placed in 12-well imaging plates (Sardtedt, 83.3921) or in 96-well imaging plates (Sigma-Aldrich, CLS3904-100EA), for 24 h prior to treatment.

    other:

    Article Title: Supporting Information Chloroacetamide-Modified Nucleotide and RNA for Bioconjugations and Cross-Linking with RNA-Binding Proteins
    Article Snippet: ZR smallRNA PAGE recovery kit was purchased from Zymo research.



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    Image Search Results


    BAITs enhance antigen uptake and digestion by DCs under TGFβ stimulation. (A) Schematic illustration of real-time DC migration assay. BAITs in the lower chamber create a chemokine gradient to attract DCs from the upper chamber. (B) Kinetics of DC migration measured as cell index (n = 3; n.s. is P > 0.05, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (C) Crystal violet staining of non-migrated DCs (upper chamber) and Calcein-AM staining of migrated DCs (lower chamber) showing enhanced DC recruitment by BAITs. (D) The antigen uptake efficiency of DCs under varying antigen concentrations with or without TGFβ (n = 5; ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (E) Heatmap of immune-related genes in DCs showing upregulation of antigen presentation and co-stimulation markers and downregulation of suppressive factors as antigen concentration increases. (F) Antigen digestion capacity of DCs assessed in the presence or absence of TGFβ (n = 8; ∗∗∗∗ is P < 0.0001 by two-tailed Student's t -test). (G) Fluorescent images showing enhanced antigen uptake in DCs mediated by BAITs (quantified on right; n = 10; ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (H) Flow cytometry analysis of antigen-positive (Cy3 + ) DCs after incubation with free antigen or BAIT (quantified on right; n = 4; ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (I) BAITs improve antigen digestion and uptake of DCs under TGFβ stimulation. DCs were first incubated with Cy5-antigens for 12 h, followed by medium replacement and subsequent treatment with Cy3-antigens or Cy3-BAITs for an additional 6 h. Data are presented as mean ± SD.

    Journal: Bioactive Materials

    Article Title: Countering postoperative immune suppression with a self-assembling dendritic cell nanovaccine

    doi: 10.1016/j.bioactmat.2026.05.005

    Figure Lengend Snippet: BAITs enhance antigen uptake and digestion by DCs under TGFβ stimulation. (A) Schematic illustration of real-time DC migration assay. BAITs in the lower chamber create a chemokine gradient to attract DCs from the upper chamber. (B) Kinetics of DC migration measured as cell index (n = 3; n.s. is P > 0.05, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (C) Crystal violet staining of non-migrated DCs (upper chamber) and Calcein-AM staining of migrated DCs (lower chamber) showing enhanced DC recruitment by BAITs. (D) The antigen uptake efficiency of DCs under varying antigen concentrations with or without TGFβ (n = 5; ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (E) Heatmap of immune-related genes in DCs showing upregulation of antigen presentation and co-stimulation markers and downregulation of suppressive factors as antigen concentration increases. (F) Antigen digestion capacity of DCs assessed in the presence or absence of TGFβ (n = 8; ∗∗∗∗ is P < 0.0001 by two-tailed Student's t -test). (G) Fluorescent images showing enhanced antigen uptake in DCs mediated by BAITs (quantified on right; n = 10; ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (H) Flow cytometry analysis of antigen-positive (Cy3 + ) DCs after incubation with free antigen or BAIT (quantified on right; n = 4; ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (I) BAITs improve antigen digestion and uptake of DCs under TGFβ stimulation. DCs were first incubated with Cy5-antigens for 12 h, followed by medium replacement and subsequent treatment with Cy3-antigens or Cy3-BAITs for an additional 6 h. Data are presented as mean ± SD.

    Article Snippet: For MHC I analysis, the cells were incubated with rat CoraLite Plus 488 anti-mouse LAMP1 antibody (1:200) and rabbit anti-mouse MHC I antibody (1:100) overnight at 4 °C, followed by Cy5-conjugated anti-rabbit secondary antibody (1:200) for 1 h at 37 °C.

    Techniques: Migration, Staining, Immunopeptidomics, Concentration Assay, Two Tailed Test, Flow Cytometry, Incubation